Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics.

نویسندگان

  • Janine Schulte-Zweckel
  • Federica Rosi
  • Domalapally Sreenu
  • Hendrik Schröder
  • Christof M Niemeyer
  • Gemma Triola
چکیده

A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.

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عنوان ژورنال:
  • Chemical communications

دوره 50 84  شماره 

صفحات  -

تاریخ انتشار 2014